Comparison of serological assays in human Middle East respiratory syndrome (MERS)-coronavirus infection.
Identifieur interne : 001857 ( Main/Exploration ); précédent : 001856; suivant : 001858Comparison of serological assays in human Middle East respiratory syndrome (MERS)-coronavirus infection.
Auteurs : Sang Won Park ; Ranawaka A P M. Perera ; Pyoeng Gyun Choe ; Eric H Y. Lau ; Seong Jin Choi ; June Young Chun ; Hong Sang Oh ; Kyoung-Ho Song ; Ji Hwan Bang ; Eu Suk Kim ; Hong Bin Kim ; Wan Beom Park ; Nam Joong Kim ; Leo Lit Man Poon ; Malik Peiris ; Myoung Don OhSource :
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [ 1560-7917 ] ; 2015.
Descripteurs français
- KwdFr :
- Adulte, Animaux, Anticorps antiviraux (sang), Anticorps neutralisants (sang), Chameaux (sang), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Femelle, Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Mâle, Test ELISA (), Tests de neutralisation ().
- MESH :
- diagnostic : Infections à coronavirus.
- immunologie : Coronavirus du syndrome respiratoire du Moyen-Orient.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- sang : Anticorps antiviraux, Anticorps neutralisants, Chameaux.
- virologie : Infections à coronavirus.
- Adulte, Animaux, Femelle, Humains, Mâle, Test ELISA, Tests de neutralisation.
English descriptors
- KwdEn :
- Adult, Animals, Antibodies, Neutralizing (blood), Antibodies, Viral (blood), Camelus (blood), Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Enzyme-Linked Immunosorbent Assay (methods), Female, Humans, Male, Middle East Respiratory Syndrome Coronavirus (immunology), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Neutralization Tests (methods).
- MESH :
- chemical , blood : Antibodies, Neutralizing, Antibodies, Viral.
- blood : Camelus.
- diagnosis : Coronavirus Infections.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Enzyme-Linked Immunosorbent Assay, Neutralization Tests.
- virology : Coronavirus Infections.
- Adult, Animals, Female, Humans, Male.
Abstract
Plaque reduction neutralisation tests (PRNT), microneutralisation (MN), Middle East respiratory syndrome (MERS)-spike pseudoparticle neutralisation (ppNT) and MERS S1-enzyme-linked immunosorbent assay (ELISA) antibody titres were compared using 95 sera from 17 patients with MERS, collected two to 46 days after symptom onset. Neutralisation tests correlated well with each other and moderately well with S1 ELISA. Moreover to compare antigenic similarity of genetically diverse MERS-CoV clades, the response of four sera from two patients sampled at two time periods during the course of illness were tested by 90% PRNT. Genetically diverse MERS-CoV clades were antigenically homogenous.
DOI: 10.2807/1560-7917.ES.2015.20.41.30042
PubMed: 26538277
Affiliations:
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Lau</name></author><author><name sortKey="Choi, Seong Jin" sort="Choi, Seong Jin" uniqKey="Choi S" first="Seong Jin" last="Choi">Seong Jin Choi</name></author><author><name sortKey="Chun, June Young" sort="Chun, June Young" uniqKey="Chun J" first="June Young" last="Chun">June Young Chun</name></author><author><name sortKey="Oh, Hong Sang" sort="Oh, Hong Sang" uniqKey="Oh H" first="Hong Sang" last="Oh">Hong Sang Oh</name></author><author><name sortKey="Song, Kyoung Ho" sort="Song, Kyoung Ho" uniqKey="Song K" first="Kyoung-Ho" last="Song">Kyoung-Ho Song</name></author><author><name sortKey="Bang, Ji Hwan" sort="Bang, Ji Hwan" uniqKey="Bang J" first="Ji Hwan" last="Bang">Ji Hwan Bang</name></author><author><name sortKey="Kim, Eu Suk" sort="Kim, Eu Suk" uniqKey="Kim E" first="Eu Suk" last="Kim">Eu Suk Kim</name></author><author><name sortKey="Kim, Hong Bin" sort="Kim, Hong Bin" uniqKey="Kim H" first="Hong Bin" last="Kim">Hong Bin Kim</name></author><author><name sortKey="Park, Wan Beom" sort="Park, Wan Beom" uniqKey="Park W" first="Wan Beom" last="Park">Wan Beom Park</name></author><author><name sortKey="Kim, Nam Joong" sort="Kim, Nam Joong" uniqKey="Kim N" first="Nam Joong" last="Kim">Nam Joong Kim</name></author><author><name sortKey="Poon, Leo Lit Man" sort="Poon, Leo Lit Man" uniqKey="Poon L" first="Leo Lit Man" last="Poon">Leo Lit Man Poon</name></author><author><name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name></author><author><name sortKey="Oh, Myoung Don" sort="Oh, Myoung Don" uniqKey="Oh M" first="Myoung Don" last="Oh">Myoung Don Oh</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26538277</idno><idno type="pmid">26538277</idno><idno type="doi">10.2807/1560-7917.ES.2015.20.41.30042</idno><idno type="wicri:Area/PubMed/Corpus">001401</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001401</idno><idno type="wicri:Area/PubMed/Curation">001401</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001401</idno><idno type="wicri:Area/PubMed/Checkpoint">001604</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001604</idno><idno type="wicri:Area/Ncbi/Merge">001338</idno><idno type="wicri:Area/Ncbi/Curation">001338</idno><idno type="wicri:Area/Ncbi/Checkpoint">001338</idno><idno type="wicri:Area/Main/Merge">001862</idno><idno type="wicri:Area/Main/Curation">001857</idno><idno type="wicri:Area/Main/Exploration">001857</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparison of serological assays in human Middle East respiratory syndrome (MERS)-coronavirus infection.</title><author><name sortKey="Park, Sang Won" sort="Park, Sang Won" uniqKey="Park S" first="Sang Won" last="Park">Sang Won Park</name><affiliation><nlm:affiliation>These authors contributed equally to the work.</nlm:affiliation><wicri:noCountry code="no comma">These authors contributed equally to the work.</wicri:noCountry></affiliation></author><author><name sortKey="Perera, Ranawaka A P M" sort="Perera, Ranawaka A P M" uniqKey="Perera R" first="Ranawaka A P M" last="Perera">Ranawaka A P M. Perera</name></author><author><name sortKey="Choe, Pyoeng Gyun" sort="Choe, Pyoeng Gyun" uniqKey="Choe P" first="Pyoeng Gyun" last="Choe">Pyoeng Gyun Choe</name></author><author><name sortKey="Lau, Eric H Y" sort="Lau, Eric H Y" uniqKey="Lau E" first="Eric H Y" last="Lau">Eric H Y. Lau</name></author><author><name sortKey="Choi, Seong Jin" sort="Choi, Seong Jin" uniqKey="Choi S" first="Seong Jin" last="Choi">Seong Jin Choi</name></author><author><name sortKey="Chun, June Young" sort="Chun, June Young" uniqKey="Chun J" first="June Young" last="Chun">June Young Chun</name></author><author><name sortKey="Oh, Hong Sang" sort="Oh, Hong Sang" uniqKey="Oh H" first="Hong Sang" last="Oh">Hong Sang Oh</name></author><author><name sortKey="Song, Kyoung Ho" sort="Song, Kyoung Ho" uniqKey="Song K" first="Kyoung-Ho" last="Song">Kyoung-Ho Song</name></author><author><name sortKey="Bang, Ji Hwan" sort="Bang, Ji Hwan" uniqKey="Bang J" first="Ji Hwan" last="Bang">Ji Hwan Bang</name></author><author><name sortKey="Kim, Eu Suk" sort="Kim, Eu Suk" uniqKey="Kim E" first="Eu Suk" last="Kim">Eu Suk Kim</name></author><author><name sortKey="Kim, Hong Bin" sort="Kim, Hong Bin" uniqKey="Kim H" first="Hong Bin" last="Kim">Hong Bin Kim</name></author><author><name sortKey="Park, Wan Beom" sort="Park, Wan Beom" uniqKey="Park W" first="Wan Beom" last="Park">Wan Beom Park</name></author><author><name sortKey="Kim, Nam Joong" sort="Kim, Nam Joong" uniqKey="Kim N" first="Nam Joong" last="Kim">Nam Joong Kim</name></author><author><name sortKey="Poon, Leo Lit Man" sort="Poon, Leo Lit Man" uniqKey="Poon L" first="Leo Lit Man" last="Poon">Leo Lit Man Poon</name></author><author><name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name></author><author><name sortKey="Oh, Myoung Don" sort="Oh, Myoung Don" uniqKey="Oh M" first="Myoung Don" last="Oh">Myoung Don Oh</name></author></analytic><series><title level="j">Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin</title><idno type="eISSN">1560-7917</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Animals</term><term>Antibodies, Neutralizing (blood)</term><term>Antibodies, Viral (blood)</term><term>Camelus (blood)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (virology)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Neutralization Tests (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Anticorps neutralisants (sang)</term><term>Chameaux (sang)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Femelle</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (virologie)</term><term>Mâle</term><term>Test ELISA ()</term><term>Tests de neutralisation ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Camelus</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Neutralization Tests</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps neutralisants</term><term>Chameaux</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Animals</term><term>Female</term><term>Humans</term><term>Male</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Animaux</term><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Test ELISA</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plaque reduction neutralisation tests (PRNT), microneutralisation (MN), Middle East respiratory syndrome (MERS)-spike pseudoparticle neutralisation (ppNT) and MERS S1-enzyme-linked immunosorbent assay (ELISA) antibody titres were compared using 95 sera from 17 patients with MERS, collected two to 46 days after symptom onset. Neutralisation tests correlated well with each other and moderately well with S1 ELISA. Moreover to compare antigenic similarity of genetically diverse MERS-CoV clades, the response of four sera from two patients sampled at two time periods during the course of illness were tested by 90% PRNT. Genetically diverse MERS-CoV clades were antigenically homogenous. </div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Bang, Ji Hwan" sort="Bang, Ji Hwan" uniqKey="Bang J" first="Ji Hwan" last="Bang">Ji Hwan Bang</name><name sortKey="Choe, Pyoeng Gyun" sort="Choe, Pyoeng Gyun" uniqKey="Choe P" first="Pyoeng Gyun" last="Choe">Pyoeng Gyun Choe</name><name sortKey="Choi, Seong Jin" sort="Choi, Seong Jin" uniqKey="Choi S" first="Seong Jin" last="Choi">Seong Jin Choi</name><name sortKey="Chun, June Young" sort="Chun, June Young" uniqKey="Chun J" first="June Young" last="Chun">June Young Chun</name><name sortKey="Kim, Eu Suk" sort="Kim, Eu Suk" uniqKey="Kim E" first="Eu Suk" last="Kim">Eu Suk Kim</name><name sortKey="Kim, Hong Bin" sort="Kim, Hong Bin" uniqKey="Kim H" first="Hong Bin" last="Kim">Hong Bin Kim</name><name sortKey="Kim, Nam Joong" sort="Kim, Nam Joong" uniqKey="Kim N" first="Nam Joong" last="Kim">Nam Joong Kim</name><name sortKey="Lau, Eric H Y" sort="Lau, Eric H Y" uniqKey="Lau E" first="Eric H Y" last="Lau">Eric H Y. Lau</name><name sortKey="Oh, Hong Sang" sort="Oh, Hong Sang" uniqKey="Oh H" first="Hong Sang" last="Oh">Hong Sang Oh</name><name sortKey="Oh, Myoung Don" sort="Oh, Myoung Don" uniqKey="Oh M" first="Myoung Don" last="Oh">Myoung Don Oh</name><name sortKey="Park, Sang Won" sort="Park, Sang Won" uniqKey="Park S" first="Sang Won" last="Park">Sang Won Park</name><name sortKey="Park, Wan Beom" sort="Park, Wan Beom" uniqKey="Park W" first="Wan Beom" last="Park">Wan Beom Park</name><name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name><name sortKey="Perera, Ranawaka A P M" sort="Perera, Ranawaka A P M" uniqKey="Perera R" first="Ranawaka A P M" last="Perera">Ranawaka A P M. Perera</name><name sortKey="Poon, Leo Lit Man" sort="Poon, Leo Lit Man" uniqKey="Poon L" first="Leo Lit Man" last="Poon">Leo Lit Man Poon</name><name sortKey="Song, Kyoung Ho" sort="Song, Kyoung Ho" uniqKey="Song K" first="Kyoung-Ho" last="Song">Kyoung-Ho Song</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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